ATAC²âÐòµÄÈ«³ÆÊÇAssay for Transposase Accessible Chromatin using sequencing,ÔËÓòâÐòÊÖ¶ÎÑо¿×ª×ùø¿É¿¿½üµÄȾɫÖʵÄÒ»ÖÖÊÖÒÕ¡£¸ÃÊÖÒÕͨ¹ýת×ùø¶ÔijÖÖÌØ׼ʱ¿ÕÏ¿ª·ÅµÄºËȾɫÖÊÇøÓò¾ÙÐÐÇи½ø¶ø»ñµÃÔÚ¸ÃÌØ׼ʱ¿ÕÏ»ùÒò×éÖлîԾת¼µÄµ÷¿ØÐòÁС£ÕâÏîÊÖÒÕ½öÐèÉÙÁ¿Ï¸°û±ã¿É»ñµÃʵʱȫ»ùÒò×é»îÐÔµ÷¿ØÐòÁÐÐÅÏ¢£¬Ó¦ÓÃÓÚת¼Òò×ÓÍŽáÆÊÎö¡¢ºËСÌ嶨λ¡¢»îÐÔµ÷¿ØÔª¼þÂþÑܵÈÑо¿£¬ÔÚ±í¹ÛÒÅ´«»úÖÆÑо¿ÁìÓòÓÐÁÉÀ«µÄÓ¦ÓÃÔ¶¾°¡£
ATAC-seqÖ÷ҪʵÑéÁ÷³ÌÊ×ÏÈÊÇÖƱ¸Ï¸°ûÐüÒº£¬È»ºóÁѽâÒºÁѽâϸ°û»ñµÃϸ°ûºË£¬½øÒ»²½Ê¹ÓÃTn5ת×ùøøÇв¢´¿»¯£¬×îºó½ÓÄÉDNAƬ¶Ï£¬¶Ô½ÓÄÉƬ¶Ï¾ÙÐÐ×îºóÐÞ¸´¡¢3¡¯¶Ë¼ÓA¡¢ÅþÁ¬²âÐòÌÖÂÛ£¬Æ¬¶Ï¾ÞϸѡÔñÒÔ¼°PCAÀ©ÔöµÈ°ì·¨»ñµÃ±ê×¼µÄ¸ßͨÁ¿²âÐòÎĿ⣬ʹÓÃIllumina²âÐòƽ̨¾ÙÐвâÐò¡£
Ôʼ²âÐòReads¾ÒÑÍùÌÖÂۺ͹ýÂ˵ÍÖÊÁ¿£¬»ñµÃClean Reads¡£½«Clean ReadsÓë²Î¿¼»ùÒò×éÐòö¾ÙÐбȶԣ¬Í¨¹ýReadsÔÚ»ùÒò×éÉϵıȶÔλÖÃÐÅÏ¢£¬¾ÙÐÐPeak calling£¬²¢¾ÙÐÐmotifÕ¹Íû¡¢×ªÂ¼Òò×ӰлùÒòÍÚ¾ò¡¢²î±ðPeakºÍMotifÖÐÆÊÎö¡£
¶¯Îï×éÖ¯£¨°üÀ¨Õæ¾ú£©¡¢Ö²Îï×éÖ¯£¨°üÀ¨ÔåÀࣩ¡¢¶³´æµÄϸ°ûÑùÆ·£¨»ù±¾ÁýÕÖËùÓÐÎïÖÖ£©¡£
a) ϸ°û£º>1¡Á106
b) ¶¯Îï×éÖ¯£º>50 mg
c) Ö²Îï×éÖ¯£º>500 mg